Automated Fluorescence Microscope for Next Generation Sequencing

Finding themselves with a suboptimal optics solution after acquiring its original developer, Volpi came to us to optimize the design and industrialize the assembly and testing of the module.

Discipline:
Next Gen. Sequencing Next Gen. Sequencing

Customer Requirements

  • An optoelectronic expert for systems engineering, industrialization, manufacturing and life cycle management
  • An established change management system
  • Expertise in tester development and build
  • Flexibility and ability to cope with strongly fluctuating demand
  • Module manufacturing in the USA

Volpi’s Solution

  • Excellent image quality for entire sequencing runs though design optimizations for improved image quality) as well as design optimizations to avoid stray light

Stable operation over entire sequencing runs (24 hours)

  • Design optimizations for improved mechanical and optoelectronic stability
  • Automatic tester development & builds
  • 100% QA

Reliable supply

  • Professional components supply chain management
  • Manufacturing in the USA in relative proximity to the instrument integrator
  • Coping with strongly fluctuating demand

Economic module cost through material and process cost reduction initiative at Volpi

Technical Features

  • Measurement principle:
    Fluorescence Imaging
  • Illumination:
    LED, number of optical channels: four , ultra-large field of view, high power LED excitation for high throughput imaging
  • Detection:
    CCD camera, imaging resolution better than 0.3 micrometer
  • Mechanical:
    x/y stage with optical closed-loop distance control with 0.1 µm precision
  • Automatic:
    Turn table for flow cells exchange
  • Consumable type:
    Sequencing flow cell
  • Number of different component types/module:
    158
  • Number of components/module:
    490

Technical Features

  • Measurement principle:
    Fluorescence Imaging
  • Illumination:
    LED, number of optical channels: four , ultra-large field of view, high power LED excitation for high throughput imaging
  • Detection:
    CCD camera, imaging resolution better than 0.3 micrometer
  • Mechanical:
    x/y stage with optical closed-loop distance control with 0.1 µm precision
  • Automatic:
    Turn table for flow cells exchange
  • Consumable type:
    Sequencing flow cell
  • Number of different component types/module:
    158
  • Number of components/module:
    490